IN· VITRO PROPAGATION OF BLACK PEPPER (Piper nigrum)

Abstract

Black pepper (Piper nigrum) is an important spice crop among minor export crops. Inconventional vegetative propagation systems of black pepper, cuttings and seeds havebeen used. But these methods provide limited quantities of planting materials, and alsospread certain pests and diseases. These problems can be overcome by adopting apropagation method through tissue culture techniques

All experiments were set up according to completely randomized design. The beststerilization was obtained in 0.1 % HgCb solution for 10 minutes immersion prior todissection of the final explants. Bud length was maximized (4.6 ±..0.84) after 8 weeks inWP medium supplemented with 2 mgll BA and I mgll Kn. The highest survival rate (78%) was observed, when surface sterilized in leaf sections of third leaf with 0.1 % HgCI2in 10 minutes followed by dipping in 90 % alcohol solution. MS medium supplementedwith 0 mgll Kn and I mgll 2,4- D was the most suitable medium for callus formation ofpepper leaf culture.

According to the experiment results, 0.1 % HgCI2 with 10 minutes was best treatment forsurface sterilization of buds. For multiplication of pepper buds, 2 mgll BA and I mg/I Kncombination was superior to other treatments. When surface sterilized in leaf sections ofthird leaf with 0.1 %HgCI2 in 10 minutes and dipped in 90 %alcohol solution was bestfor pepper leaf culture and MS medium supplemented with 0 mg/I Kn and I mg/I 2,4- Dwas superior treatment for callus formation.