Characterization of Laccase from the Fungi Fusarium Isolated from Potato Peels Using Carbon and Nitrogen Sources

Authors

  • Joseph Minari university of lagos
  • Esther Esesoa Agho CELL BIOLOGY AND GENETICS, UNIVERSITY OF LAGOS
  • Ekenem Emelumadu
  • Oloruntimileyin Adeniyi CELL BIOLOGY AND GENETICS, UNIVERSITY OF LAGOS
  • Funke Ruth Olajiga CELL BIOLOGY AND GENETICS, UNIVERSITY OF LAGOS

DOI:

https://doi.org/10.31357/ait.v2i2.5400

Keywords:

Laccase; Guaiacol; Potato peels; Carbon source; Nitrogen source

Abstract

Laccases (E.C. 1.10.3.2 benzenediol: oxygen oxidoreductase) are an interesting group of N glycosylated multicopper blue oxidase enzymes. They are widely found in fungi, bacteria plants, insects, and lichen. They catalyze the oxidation of various phenolic and non-phenolic compounds, with the concomitant reduction of molecular oxygen to water. Laccase has various applications in industries such as textile dye bleaching, paper, and pulp bleaching, food processing, bioremediation, biodegradation, wood processing, and pharmaceuticals. However, the high cost of production has been a major hindrance to its commercial usage. This study was carried out to investigate the extraction, purification, and characterization of laccase from fungi isolated from potato peels using three different substrates. Extraction was carried out using submerged fermentation, with glucose, lactose, and maltose as the carbon sources and varying nitrogen sources; yeast and ammonium chloride (NH4Cl) Laccase was also characterized by assessing parameters such as pH, temperature, and protein concentration. Enzyme activity for maltose (yeast), glucose (yeast), glucose (NH4Cl) and lactose (NH4Cl) increased from 25oC -45 oC with optimum pH of 6,6,8 and 5 respectively while activity for maltose (NH4Cl) and lactose(yeast) increased from 25oC-65oC with optimum pH at 5 and 8 respectively. This study suggests that increased laccase production from potato peels can be achieved by using maltose, glucose and lactose as carbon sources with NH4Cl as nitrogen source.

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Published

2022-05-25

How to Cite

Minari, J., Agho, E. E., Emelumadu, E., Adeniyi, O., & Olajiga, F. R. (2022). Characterization of Laccase from the Fungi Fusarium Isolated from Potato Peels Using Carbon and Nitrogen Sources. Advances in Technology, 2(2), 183–197. https://doi.org/10.31357/ait.v2i2.5400

Issue

Volume 2, Issue 2 (2022)

Section

Emerging Food Technology

Categories

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Copyright (c) 2022 joseph minari, ESTHER ESEOSA AGHO, EKENEM EMELUMADU, Oloruntimileyin Adeniyi, Funke Ruth Olajiga

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