@article{Kokilananthan_Bulugahapitiya_Manawadu_Gangabadage_2022, title={Effect of Extraction Techniques on Phytochemicals and Antioxidants Activity of Garcinia quaesita Leaves}, volume={2}, url={https://journals.sjp.ac.lk/index.php/ait/article/view/5444}, DOI={10.31357/ait.v2i1.5444}, abstractNote={<p style="text-align: justify;"><span class="fontstyle0">Garcinia quaesita </span><span class="fontstyle2">is an endemic plant in Sri Lanka with a wide array of domestic and medicinal values, yet very limited</span> <span class="fontstyle2">studies have been reported. The goal of this study was to investigate the phytochemicals and antioxidants capacity of</span> <span class="fontstyle2">leaves of </span><span class="fontstyle0">G. quaesita </span><span class="fontstyle2">under different extraction techniques in order to monitor the effect of extraction techniques on the</span> <span class="fontstyle2">above parameters. Water was used as the solvent and four extraction methods, namely sonication (EN01, one-hour,</span><br /><span class="fontstyle2">RT, 40 kHz), Soxhlet (EN02, six-hours, 105 ºC), maceration with agitation (EN03, six-hours, RT, 1000 rpm), and</span> <span class="fontstyle2">maceration with agitation upon heating (EN04, six-hours, 60 ºC, 1000 rpm), were applied. Phytochemical screenings</span> <span class="fontstyle2">and quantification of polyphenolics (TPC), flavonoids (TFC), tannins (TTC), terpenoids (TC), saponins (SC), and</span> <span class="fontstyle2">alkaloids (AC) were performed using standard methods, whereas antioxidants capacity was assessed using FRAP and</span> <span class="fontstyle2">DPPH assays. The results revealed that </span><span class="fontstyle0">G. quaesita </span><span class="fontstyle2">contains a wide range of phytochemicals and the TPC, TTC and SC</span> <span class="fontstyle2">profiles appeared to be higher in the extraction process of EN04 (60.73 ± 0.06 mg GAE/g, 60.12 ± 0.06 mg TAE/g, and</span> <span class="fontstyle2">257.64 ± 0.72 mg SE/g, respectively) whereas those were lower in the technique EN01. In addition, TFC and TC levels</span> <span class="fontstyle2">were high in EN02 (3.02 ± 0.00 mg QE/g and 17.75 ± 0.04 mM LE/g respectively) and besides, TFC was low in method</span> <span class="fontstyle2">EN03, whereas TC was low in EN01. Interestingly, AC was found to be higher in EN03 (1.16 ± 0.03 mg AE/g) and</span> <span class="fontstyle2">lesser in EN02. Total antioxidant capacity ( FRAP value) was calculated to be higher in extraction method EN04 (72.08</span> <span class="fontstyle2">± 0.00 mg Trolox Eq/g) whereas it was lower in extraction method EN01. The method, EN03 gave the lower IC50 value</span> <span class="fontstyle2">in DPPH radical scavenging assay (10.49 ± 0.12 mg/ml), indicating high scavenging activity. In conclusion, the</span> <span class="fontstyle2">extraction technique EN04 is well suitable to extract polyphenolics and antioxidants from </span><span class="fontstyle0">G. quaesita leaves. </span><span class="fontstyle2">Though </span><span class="fontstyle0">G.</span> <span class="fontstyle0">quaesita </span><span class="fontstyle2">leaves contain a diverse range of bioactive compounds and antioxidants properties, it is important to choose</span> <span class="fontstyle2">the correct extraction technique based on the objectives of the desired research work, as the number of phytochemicals</span> <span class="fontstyle2">extracted, and antioxidant capacity varies depending on the extraction technique used.</span></p> <p><span class="fontstyle2"><em>Full paper submission of ICIET 2021</em></span></p>}, number={1}, journal={Advances in Technology}, author={Kokilananthan, Shanthirasekaram and Bulugahapitiya, Vajira and Manawadu, Harshi and Gangabadage, Chinthaka S.}, year={2022}, month={Apr.}, pages={18–30} }