SHOOT INITIATION AND MULTIPLICATION OF CORSICAN PINE IN VITRO

Abstract

From different combinations of basal medium and growth regulators tested, itwasfound that the best niediumfor producing shoots front mature embryos wasAI."'I 2 ppnt BAP + 2 ppnt NAil -1 I. 25 pplll IIIlI; front apical tneristems' CD +I. 12 pplll !:fA P + 0.0./ pptn 2, .J-D r- 0.01 pplll J.:; andfrom needle fascicles, cowith no growth regulator. Of 3 substrates tested for growing seedlings toproduce explants, the best (jiJl' apical-meristem explants) was low-nutrientcompost, and the best age o/' seedling was 2 I days. Explants from one-year oldplants and shoots produced in vitro gave more shoots than did those frotnmature trees; there was no significant difference in the length of the shoots.Production of adventitious budsfor rapid multiplication was greater on exp Iants0/ apical meristem origin than on those front mature embryos or needle[ascicles; the percentage survival decreased significantly in second and thirdgenerations ofexplants oral! three origins.