IN VITRO MULTIPLICATION AND ROOTING OF Artocarpus heterophyllus LAM

Abstract

Artocarpus heterophyllus is an out breeding species. Therefore germination or seeds maygive seedlings with vast genetic diversity. Clonal propagation of selected genotypes is veryuseful in maintaining selected characters.

Apical meristem from 20-30 years old jak plants were cultured in modified Campbell andDurzan (CD) medium supplemented with 0.5mg/l Indole Butyric Acid (IBA) in order toelongate shoots ill vitro. After six weeks of incubation in 25± I(lC ( 16 hr day). 60% of theexplants produced elongated shoots. They were cultured on Murashige & Skoog (MS)medium containing l.5mg/1 IBA and a.5mgll kinetin for shoot multiplication and 4-5 shootsper explant were obtained after live weeks of incuhation. Non-multiplied shoots weretransferred into '12 MS medium containing 1.5mg/l IBA and I.Omg/l Naphthalene AceticAcid (NAA) after five weeks. If shoots were kept in the same medium ('12 MS mediumcontaining 1.5mg/l IBA + I.Omg/l NAA) for more than two weeks they will produce callusinstead or roots at the hase. Therefore after two weeks they were transferred into CDmedium containing a.5mgll IBA for rooting. Root initials were observed after Ia days ofincuhation.Proceedings