Qualitative Phytochemical Screening, Anti-Bacterial Activity and TLC Profiling of Different Parts of Three Medicinal Plants

Abstract

The aim of this study was to carry out phytochemical screening Anti-bacterial activity and TLC profiling of sequentially extracted petroleum ether, dichloromethane, ethyl acetate and methanol extracts of leaf and barks of the Nerium oleander, Leaf, bark and seeds of Cascabela thevetia and Cerbera odollum. The phytochemical analysis of the extracts obtained from different solvents was carried and Ant-bacterial activity was carried out for all the extractions at 25 mg/ml and 50 mg/ml test concentration against E.coli and S.aureus bacteria by well diffusion method. All the tested extractions showed low activity against both bacteria at 25mg/ml compared with 50mg/ml. Results were subjected to one way (ANOVA) and followed by Tukey’s test (P < 0.05). All the extractions of all plant materials showed in bioactivity against E.coli and S.aureus bacteria at 50 mg/ml among them. The statistical analysis revealed that anti-bacterial activity of the ethyl acetate extracts of C.thevetia leaves and N.oleander barks against E.coli bacteria and Methanol extract of Nerium oleander leaves and ethyl acetate extracts of C.thevetia bark against S.aureus bacteria were comparable with the positive control Amoxicillin. TLC profiling was carried out using a various solvent system of varying polarity for all sequential extractions and obtained different retention factor (Rf) values of different phytochemicals.

KEYWORDS: Phytochemical analysis, Antibacterial activity, Thin-layer chromatographic profiling, Sequential extractions, Cascabela thevetia and Cerbera odollum